Functional Assay:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
Selection:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
Marker:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
TA Cloning:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
Plasmid Preparation:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
Clone Assay:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
Construct:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
Sequencing:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
DNA Sequencing:Article Title: Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation
Article Snippet: Invitrogen E. coli DH10B F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74 deoR recA1 endA1 araD139 D(ara, leu)7697 galU galK -rpsL nupG Invitrogen H. influenzae A2 Type b strain Ref. 24 H. influenzae 2019 Non-typeable strain Ref. 57 H. influenzae A2STF siaA This study H. influenzae A2STFC.P4 siaA , plus a functional siaA This study H. influenzae A2STFIRA siaA This study H. influenzae A2SB siaB This study H. influenzae 276.4 lsgE Ref. 26 H. influenzae 276.4STF lsgE , siaA This study H. influenzae A2L3A lic3A This study H. influenzae A2STFL3A siaA , lic3A This study H. influenzae A2lsgB lsgB This study H. influenzae A2STFL3AlsgB siaA , lic3A , lsgB This study Plasmids Selection marker and description Source or reference pCR2.1 Ampicillin, kanamycin, TA cloning vector Invitrogen pBluescript KS II Ampicillin, cloning vector Stratagene pHS89–21 Ampicillin, kanamycin, siaA cloned into pCR2.1 This study pBSL15 Ampicillin, kanamycin, used as a backbone for pABR3 and pHiCM1 Ref. 58 pBSL86 Ampicillin, kanamycin, source of the kanamycin cassette for pHS89–106K6 Ref. 58 pABR3 Ampicillin, spectinomycin, pBSL15 with a spectinomycin cassette Ref. 58 pSiaB2 Ampicillin, kanamycin, siaB cloned into pCR2.1 This study pSiaB2Spec Ampicillin, kanamycin, spectinomycin, pSiaB2 with a spectinomycin cassette in siaB This study pHS89–103 Ampicillin, siaA from pHS89–21 cloned into pBluescript KS II This study pHS89–103K6 Ampicillin, kanamycin, pHS89–103 with a kanamycin cassette in siaA This study pTAV1 Ampicillin, TA cloning vector constructed from pBluescript KS II Ref. 59 pHiCM1 Ampicillin, chloramphenicol, pBSL15 with chloramphenicol cassette This study pIRA Ampicillin, intergenic region from NTHi 2019 cloned into pTAV1 This study pIRACM Ampicillin, chloramphenicol, pIRA with chloramphenicol cassette This study pCMR Ampicillin, kanamycin, chloramphenicol, source of chloramphenicol cassette Ref. 35 p0352EX Ampicillin, kanamycin, lic3A gene cloned into pCR2.1 This study p0352EXCM Ampicillin, kanamycin, chloramphenicol, p0353EXCM with a chloramphenicol cassette in lic3A This study PGEMLOS2 Ampicillin, lsgA, lsgB, lsgC, and lsgD cloned into pGEM3Zf This study pRSM1775 Ampicillin, kanamycin, chloramphenicol, source of a nonpolar chloramphenicol cassette This study pGEMLOSABCD Ampicillin, lsgA, B, C, D cloned into pGEM3zf This study pGEMLOS2ABCDerm Ampicillin, erythromycin, pGEMLOSABCD with an erythromycin cassette in lsgB This study pSAIRCM Ampicillin, kanamycin, chloramphenicol, complementation construct containing the intergenic sequence, chloramphenicol gene, and siaA This study by guest on N ovem ber 15, 2015 http://w w w .jbc.org/ D ow nloaded from automated sequencer using fluorescent terminator dye tags at the DNA Sequencing Facility (University of Iowa).
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